Ratios for those with HSV-1 or HSV-2 antibody types were not affected by disease expression. Testing options for herpes simplex virus (HSV) have expanded in the United. Ratios of HSV-2 to HSV-1 binding were higher in asymptomatic versus symptomatic patients with dual antibody (P less than 0.01). 2 Because most persons with antibody to HSV-2 are unaware of their infection, 37 public health programs to control genital herpes will likely require efforts to identify. With HSV-1, the reverse was true: commercial tests often overlooked HSV-1 antibodies later found with the. 1 Recently, some authorities have advocated a national HSV-2 prevention campaign to diminish this burden of disease. Researchers from the University of Washington in Seattle and the Westover Heights Clinic in Portland, OR found that patients with low positive HSV-2 results on commercial EIAs frequently tested negative with the more sensitive Western Blot. Typing results were confirmed by cross-adsorption of sera ("adsorption blot assay"). AN ESTIMATED 22 OF PERSONS in the United States are infected with herpes simplex virus type 2 (HSV-2). Sera from 52 patients with asymptomatic HSV-1, HSV-2 or dual infection were also tested by WB. Methods: We used de-identified HSV Western Blot data performed at the University of Washington Virology Laboratory between 1999-2020. Densitometry was shown to amplify and clarify the band corresponding to the HSV-2 specific glycoprotein gG-2 and was useful, in this respect, for detecting HSV-2 antibody in the presence of HSV-1 antibody. However, ratios on sera with HSV-1 antibody or dual antibody status showed considerable overlap. Binding ratios accurately identified patients with primary HSV-2 infections. To determine the clinical correlation of these ratios, sera from 81 patients with culture-proven oral or genital herpes were tested by Western blot (WB) and densitometry. By densitometry, differential binding of total antibody on HSV-1 versus HSV-2 strips can be expressed as a ratio. It’s still a blood test at the end of the day. I just feel like, if this is such a crazy and unpredictable virus, how is this test so much better than others. A predominance of antibody binding to either the HSV-1 or the HSV-2-containing blot has been reported to indicate infection with HSV-1 or HSV-2, respectively. Has anyone ever heard of a false positive western blot I know this test is considered the gold standard. Western blot assays have been described for detecting antibodies to herpes simplex virus (HSV).
0 kommentar(er)
